顺铂对人肺癌LTEP-a-2细胞DNA损伤相关基因表达的影响

The Effect of Cisplatin on the Expression of Genes Involved in DNA Damage Response in Human Lung Cancer Cell Line LTEP-a-2

作者: 专业:肿瘤学 导师:束永前 年度:2009 学位:硕士  院校: 南京医科大学

Keywords

cisplatin, lung cancer cell, DNA damage

        目的:研究顺铂对人肺癌LTEP-a-2细胞DNA损伤相关基因表达的影响。方法:1.用CCK-8法检测顺铂对人肺癌细胞LTEP-a-2增殖的影响。2. PI单标流式细胞术检测顺铂对LTEP-a-2细胞周期和凋亡的影响。3. PCR Assay检测顺铂对LTEP-a-2细胞DNA损伤相关基因表达的改变。结果:1.顺铂抑制LTEP-a-2细胞的增殖。2.顺铂作用后,LTEP-a-2细胞被阻滞于G2/M期,并诱导细胞发生凋亡。3.顺铂作用后LTEP-a-2细胞的DNA损伤相关基因表达发生改变,PCBP4、TP73基因表达上调,DDB1、LIG1基因表达下调。结论:顺铂影响LTEP-a-2细胞的DNA损伤相关基因表达,其作用机制与影响DNA损伤相关基因有关。
    ObjectiveTo investigate the effect of cisplatin on the expression of genes involved in DNA damage response in human lung cancer cell line LTEP-a-2. Methods1. The effect of cisplatin on the growth of human lung caner cell line LTEP-a-2 was studied with CCK-8 assay.2. The effect of cisplatin on the cell cycle phase distribution and apoptosis in LTEP-a-2 cells was determined using flow cytometry by a propidium iodide method.3. The effect of cisplatin on the expression of genes involved in DNA damage response in human lung cancer cell line LTEP-a-2 was studied with PCR assay.Results1. Cisplatin inhibited the proliferation of LTEP-a-2 cells.2. Flow cytometric analyses showed that LTEP-a-2 cells treated with cisplatin were accumulated in G2/M phase in cell cycle.3. The expression of genes involved in DNA damage response changed in LTEP-a-2 cells treated with cisplatin,The expressions of PCBP4 and TP73 were upregulated, while DDB1 and LIG1 were downregulated. ConclusionThe expressions of genes involved in DNA damage response changed in LTEP-a-2 cells treated with cisplatin. The effect of cisplatin on the expression of genes involved in DNA damage response may be corrected with the mechanism of action of cisplatin.
        

顺铂对人肺癌LTEP-a-2细胞DNA损伤相关基因表达的影响

中英文缩略词表4-5
中文摘要5-6
英文摘要6-7
前言8-10
材料与方法10-18
结果18-24
分析与讨论24-28
小结28-29
参考文献29-33
综述33-48
    参考文献41-48
在读期间发表的文章48-49
致谢49
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