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MIF-173位点单核苷酸多态性与中国苏皖地区汉族人群膀胱癌关系的研究

Macrophage Migration Inhibitory Factor Gene -173G>C Polymorphism and Risk of Bladder Cancer in Southeast China: a Case-control Analysis

作者: 专业:泌尿外科 导师:张炜 年度:2009 学位:硕士  院校: 南京医科大学

Keywords

MIF, polymorphism, bladder cancer, molecular epidemiology

        目的研究巨噬细胞移动抑制因子(MIF)基因-173位点单核苷酸多态性与中国南方汉族人群膀胱癌的关系。方法采用病例-对照研究,从325例膀胱癌患者(病例组)和345例非肿瘤、非膀胱疾病患者(对照组)外周血中提取基因组DNA,应用聚合酶链反应限制性片段长度多态性( PCR-RFLP)方法分析病例组和对照组的MIF基因- 173位点的多态性。对收集的47份膀胱癌手术组织标本和1份正常膀胱组织标本石蜡切片行实验用鼠抗人多克隆抗体MIF(工作浓度1:100)免疫组化染色,分析肿瘤细胞染色结果。结合免疫组化结果,分析-137位点不同基因型与膀胱癌易感性的关系。结果携带MIF-173*C等位基因的个体发生膀胱癌的风险性是G/G基因型的0.57倍。在老年人(年龄>70岁)、男性、吸烟者和曾饮酒者人群中,MIF - 173*C等位基因携带者罹患膀胱癌的危险性显著降低(老年人OR=0.56,95% CI: 0.39-0.81;男性OR=0.47,95% CI: 0.33-0.68;吸烟者OR=0.54,95% CI: 0.35-0.85;曾饮酒者OR=0.44,95% CI: 0.27-0.71)。,MIF的蛋白表达(核和胞浆染色阳性)在MIF-137GG型和GC/CC型的膀胱癌组织之间存在明显的差异(在GG型和GC/CC型两组中,MIF强染色分别占39.1%和75.0% ,P = 0.028)。结论MIF-173*C等位基因可能与我国南方汉族人群膀胱癌发生有关。
    ObjectiveThis study aimed to assess the association between the MIF-173 polymorphism and bladder cancer risk. We hypothesized that this polymorphism may contribute to genetic susceptibility to bladder cancer.MethodsIn a hospital-based case-control study of 325 patients with bladder cancer and 345 cancer-free control subjects frequency-matched by age and sex, we genotyped the MIF polymorphism and analyzed immunohistochemical stained operational bladder cancer tissue sections for MIF.ResultsWe found that individuals with GC/CC genotypes had a significantly decreased risk of bladder cancer (adjusted OR = 0.57, 95% CI, 0.41-0.79) than those with GG genotype. In the stratification analysis, we found that the decreased risk was more pronounced among older subjects (0.56, 0.39-0.81), men (0.47, 0.33–0.68), smokers (0.54, 0.35-0.85), and ever-drinkers (0.44, 0.27-0.71). The percentage of positive staining in the cytoplasm and nucleus in the normal and bladder cancer with CC /GC genotype tissues was higher than that of GG genotype bladder cancer tissue.ConclusionsOur data suggest that MIF -173G>C polymorphism may play a role in the etiology of bladder cancer in southern Chinese populations.
        

MIF-173位点单核苷酸多态性与中国苏皖地区汉族人群膀胱癌关系的研究

缩略词中英文对照4-5
中文摘要5-7
英文摘要7-8
前言9-13
材料与方法13-22
结果22-27
讨论27-30
小结30-31
参考文献31-36
综述36-47
    参考文献42-47
附录47-57
攻读硕士学位期间撰写论文57-58
发表论文58-66
致谢66
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