Runx1基因mRNA表达与非小细胞肺癌发生及淋巴结转移的相关性研究
Association Study of mRNA Expression of Runx1 Gene with Tumorigenesis and Lymphatic Metastasis in Human Non-small Cell Lung Cancer
作者： 吴卫兵 专业：胸外科 导师：陈亮 年度:2009 学位：硕士 院校： 南京医科大学
Keywords
Runx1 gene, non-small cell lung cancer, tumorigenesis, lymphatic metastasis
目的:本实验探讨Runx1基因mRNA表达与人非小细胞肺癌(NSCLC)发生、发展的关系,为非小细胞肺癌诊断治疗探索新的可能的靶点。方法:1本组选取组织标本均取自南京医科大学第一附属医院(江苏省人民医院)胸外科2007年12月至2008年4月的临床手术标本,取37例NSCLC标本,1O例肺良性病变旁正常肺组织,应用逆转录-多聚合酶链反应(RT—PcR)的方法检测NSCLC和正常对照组织中Runx1的mRNA表达水平,2统计学分析:采用SPSS11.0统计软件分析检验.结果:肺良性病变旁正常肺组织和肺癌肿瘤组织中均有Runx1基因表达,肺癌肿瘤组织中Runx1基因表达水平显著低于肺良性病变旁正常肺组织(P<0.01),差异有统计学意义,肺癌组织中Runx1基因表达水平降低与淋巴结转移相关(P<0.05),差异有统计学意义,与肺癌组织学类型、细胞分化程度以及患者的年龄、性别无关(P>0.05)。结论:与良性病变旁正常肺组织相比,Runx1基因的mRNA水平在肿瘤组织中表达下降,并且与淋巴结转移相关,提示其可能参与肺癌的发生发展过程,为非小细胞肺癌诊断治疗探索新的可能的靶点。
Objective: To investigate mRNA expression of human runt-related transcription factor1(Runx1) in non small cell lung cancer(NSCLC), and its genesis and development.Methods: The mRNA expression of Runx1 was measured by semi-quantitative RT-PCR in 37 cases of NSCLC tissue, and 10 cases of lung benign lesion tissue. Results: The mRNA expression level of RunX1 gene was significantly lower in NSCLC tissues than normal tissues (P<0.01). The mRNA expression level of Runx1 gene was significantly related to lymphatic metastasis (P<0.05).Conclusion: Compared with the control, the expression of Runx1 mRNA was reduced and was concerned with lymph node metastasis. It suggest that Runx1 may be involved in the generation and development of NSCLC.
Runx1基因mRNA表达与非小细胞肺癌发生及淋巴结转移的相关性研究
| 1. 缩略语 | 4-5 |
| 2. 中文摘要 | 5-7 |
| 3. 英文摘要 | 7 |
| 4. 前言 | 8-11 |
| 5. 材料与方法 | 11-17 |
| 6. 结果 | 17-21 |
| 7. 分析与讨论 | 21-26 |
| 8 小结 | 26-27 |
| 9. 参考文献 | 27-31 |
| 10. 综述及参考文献 | 31-49 |
| 参考文献 | 43-49 |
| 11. 攻读学位期间发表文章情况 | 49-59 |
| 12. 致谢 | 59 |
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