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自由人槭(Acer×freemanii)组培快繁技术体系的构建

The Establishment of Micropropagation Technical System of Acer ×freemanii

作者: 专业:园林植物与观赏园艺 导师:苑兆和 年度:2010  院校: 山东农业大学

Keywords

        本文以自由人槭‘冷俊’( Acer×freemanii‘Marmo’)和‘秋焰’( Acer×freemanii‘Autumn Blaze’)带腋芽茎段为外植体,对其组织培养进行了系统的研究。旨在建立可用于工厂化繁殖的自由人槭微体快繁技术体系,并为其分子育种及相关研究提供技术性支持。本文对外植体的取材部位、取材时间、不同灭菌方案的灭菌效果、外植体的褐变控制方法、自由人槭组织培养的最佳启动、增殖、生根培养基等进行了系统、细致地研究。主要结论如下:1.自由人槭‘冷俊’和‘秋焰’组织培养的最佳取材时间是4月下旬~5月份。2.自由人槭‘冷俊’和‘秋焰’的组织培养的最佳取材部位均为新枝的第三节和第四节芽位。3.以5月份的带腋芽茎段为外植体,自由人槭最佳灭菌处理方案为75%酒精20s+0.1%升汞4 min。4.自由人槭‘冷俊’和‘秋焰’组织培养的最佳启动培养基均为MS空白培养基。5.自由人槭‘冷俊’和‘秋焰’,褐变发生情况相似,不同芽位的褐变度均呈“V”型变化,且峰值都出现在第3节芽位。PVP 1.0mg/L为‘冷俊’的最佳褐变抑制剂;PVP 0.5mg/L为‘秋焰’的最佳褐变抑制剂。6.植物生长调节剂对自由人槭不同品种组培苗的增殖有着不同的影响。但TDZ都是诱使‘冷俊’和‘秋焰’增殖的最重要因素;NAA不是影响组培苗增殖的主要因素;MS+TDZ 0.02mg/L是自由人槭‘冷俊’的最佳增殖培养基;MS+TDZ 0.05mg/L是自由人槭‘秋焰’的最佳增殖培养基。7. NAA对自由人槭组培苗的生根有着相似的影响,且生根现象显著,活性炭AC也起了重要作用。MS+NAA 0.1 mg/L+AC 0.5g/L为自由人槭‘冷俊’的最佳生根培养基,MS+NAA 0.2 mg/L+AC 0.6g/L为自由人槭‘秋焰’的最佳生根培养基。
    Acer×freemanii stem with axillary bud was used as explant,tissue culture of Acer×freemanii was studied systematically in the article in order to set up the technique for commercial production of Acer×freemanii and provide technical support for molecular breeding and correlation study of Acer×freemanii.During the establishment of tissue culture technique system, Acer×freemanii‘Marmo’,‘Autumn Blaze’stem with axillary bud was used as explant. In this study,the nodal position, collection time, the degerming results using different germicidal treatment programs, the control of browning method, the optimal initiation medium,the optimal proliferation medium, the optimal rooting medium in Acer×freemanii’s tissue culture were studied systematically. The results indicated that:1) The optimal collection time was the last ten days of April to May.2) The optimal nodal position was the third and the forth nodes of the new-born stem.3) Drawing the explant in May, the optimal germicidal treatment program was: 75% C2H5OH 20s+0.1% Hgcl2 4min.4) The optimal initiation medium in tissue culture of‘Marmo’and‘Autumn Blaze’was MS.5) The browning degree in‘Marmo’and‘Autumn Blaze’was similar, they were“V”type change in the browning degree with the different bud location and all the peak amplitude was the fourth bud. The optimal browning inhibitor about‘Marmo’was PVP1.0mg/L,and the optimal browning inhibitor about‘Autumn Blaze’was PVP 0.5 mg/L.6) There were different effect tendency of plant growth regulating substance on the proliferation of different Acer×freemanii plantlets,but TDZ was the dominating effect factor to proliferation of plantlets, NAA was not the dominating effect factor.The optimal proliferation medium of‘Marmo’was MS+TDZ 0.02mg/L ,the optimal proliferation medium of‘Autumn Blaze’was MS+TDZ 0.05mg/L.7) The effect of NAA on rootage of different Acer×freemanii plantlets was significant, the effect of AC was very important too. The optimal rooting medium of‘Marmo’was MS+NAA 0.1 mg/L+AC 0.5g/L, The optimal rooting medium of‘Autumn Blaze’was MS+NAA 0.2 mg/L+AC 0.6g/L.
        

自由人槭(Acer×freemanii)组培快繁技术体系的构建

摘要7-9
Abstract9-10
1 引言11-24
    1.1 木本植物组织培养研究进展11-17
        1.1.1 国外研究进展13-16
        1.1.2 国内研究进展16-17
    1.2 木本植物组织培养过程中存在的问题17-22
        1.2.1 木本植物组织培养中的培养基问题17-19
        1.2.2 木本植物组织培养中的褐变问题19-21
        1.2.3 木本植物组织培养中的生长调节剂问题21-22
    1.3 槭树科植物组织培养研究进展22-24
        1.3.1 槭树科植物组织培养研究进展23
        1.3.2 自由人槭组织培养的研究进展23-24
    1.4 本试验研究的目的与意义24
2 材料与方法24-29
    2.1 材料24
    2.2 方法24-28
        2.2.1 启动培养24-26
        2.2.2 增殖培养26-28
        2.2.3 生根培养28
    2.3 数据统计与分析方法28-29
3 结果与分析29-47
    3.1 启动培养29-35
        3.1.1 取材时期对外植体启动的影响29-30
        3.1.2 取材部位对外植体启动的影响30-32
        3.1.3 最佳消毒方案的选择32-33
        3.1.4 基本培养基对外植体启动的影响33-34
        3.1.5 不同抗褐变添加物对外植体启动的影响34-35
    3.2 增殖培养35-43
        3.2.1 植物生长调节剂对组培苗增殖的效应35-39
        3.2.2 最优增殖方案的选择39-43
    3.3 生根培养43-47
        3.3.1 不同因素对外植体生根的影响44-46
        3.3.2 不同培养基上组培苗的生根状况统计46-47
4 讨论47-54
    4.1 ‘冷俊’和‘秋焰’组织培养中的共性47-53
        4.1.1 启动培养47-50
        4.1.2 增殖培养50-52
        4.1.3 生根培养52-53
    4.2 ‘冷俊’和‘秋焰’组织培养中的个性53-54
        4.2.1 抑制褐变添加物浓度对自由人槭启动的效应53
        4.2.2 TDZ 浓度对自由人槭增殖的效应53-54
        4.2.3 NAA 浓度对自由人槭生根的效应54
5 结论54-56
    5.1 基本结论54-55
    5.2 本论文的创新点55-56
参考文献56-70
图版说明70-72
致谢72-73
攻读硕士学位期间发表的学术论文目录73
        下载全文需73


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