Computing methods for icosahedral and symmetry-mismatch reconstruction of viruse

本文刊于:《Chinese Physics B》 2018年第5期

关键词:
cryo-electron microscopy icosahedral vir

Keywords
cryo-electron microscopy;icosahedral virus;computational method;three-dimensional reconstruction;symmetry-mismatch reconstruction
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摘要
     Three-dimensional(3 D) reconstruction of icosahedral viruses has played a crucial role in the development of cryoelectron microscopy single-particle reconstruction, with many cryo-electron microscopy techniques first established for structural studies of icosahedral viruses, owing to their high symmetry and large mass. This review summarizes the computational methods for icosahedral and symmetry-mismatch reconstruction of viruses, as well as the likely challenges and bottlenecks in virus reconstruction, such as symmetry mismatch reconstruction, contrast transformation function(CTF)correction, and particle distortion.


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