首页 > 硕士 > 理学 > 正文

小麦遗传图谱的加密和籽粒可溶性糖含量的QTL分析

Increasing Density of Wheat Genetic Linkage Map and QTL Analysis for Soluble Saccharides Contents in Grains of Wheat

作者: 专业:遗传学 导师:李斯深 年度:2010 学位:硕士  院校: 山东农业大学

Keywords

Wheat, SSR, Molecular marker, Genetic map, Solublesaccharides

        高密度遗传图谱的构建对小麦遗传研究具有重要意义。微卫星或简单重复序列(SSR)多态性好,稳定性高、操作简单,通常表现为共显性,被广泛应用于小麦遗传图谱的绘制。本研究以实验室原有小麦分子标记遗传图谱为基础,对图谱中密度较小染色体进行了SSR标记补充。同时,采用高效液相色谱法对影响小麦籽粒品质的可溶性糖组分进行了鉴定和含量测定,结合加密后遗传图谱对小麦籽粒中可溶性糖含量进行了QTL分析。主要结果如下:(1)从GrainGenes上查找到461对SSR引物,主要包括Xcfa、Xcfd、Xbarc、Xgwm、Xwmc系列。利用RIL群体亲本检测到123对多态性引物。其中,Xbarc、Xgwm、Xwmc引物分别为78对、9对、2对。(2)利用RIL群体对Xbarc、Xgwm、Xwmc引物进行多态性检测,共检测出多态性引物26对,并将34个多态性位点中23个绘制在1B、2A、2D、3A、3B、5A、5B、6A、6B、6D和7A等11条染色体的遗传图谱上。(3)对小麦籽粒中可溶性糖组分分析,共鉴定出果糖、葡萄糖、蔗糖、麦芽糖和棉籽糖等5种可溶性糖。(4)可溶性糖含量的QTL分析表明,共检测到果糖、蔗糖、麦芽糖和棉籽糖等4种可溶性糖含量的13个加性QTL,位于1B、1D、2B、2D、3B、4A、4B、5A、5D、6B、7B等11条染色体,可解释表型变异的4.97%-53.57%。
    A high-density genetic map is indispensable for wheat genetic research. Microsatellite DNA markers were widely used in genetic linkage map of wheat, because of its merits of simple manipulation, good stability, codominant and high polymorphism. Chromosomes with less molecular markers on the wheat molecular genetic map which already has been constructed were counted. SSR markers from GrainGenes were found online. The polymorphism of primer pairs were detected using recombinant inbred lines (RILs) and their parents, and the marker loci were mapped using RIL population and Mapmaker/Exp3.0 software.The component and contents of soluble osaccharides in wheat were studied by high performance liquid romatography (HPLC). QTLs of soluble osaccharides contents were mapped using the RILs of wheat. The main results were as follows:(1) A total of 461 SSR pairs from GrainGenes, including Xcfa、Xcfd、Xbarc、Xgwm and Xwmc, were found online. The polymorphism of 123 SSR pairs, 78 Xbarc pairs, 9 Xgwm pairs and 2 Xwmc pairs, were detected using the parents of RILs.(2) Using our RIL population, the polymorphism of 26 SSR primer pairs with 34 loci was detected, and 23 of those loci were integrated on 11 chromosomes on our wheat genetic map, 1B、2A、2D、3A、3B、5A、5B、6A、6B、6D and 7A.(3) A total of five soluble osaccharides, Fructose, Sucrose, Maltose, and Raffinose, were found in grains of wheat by high performance liquid romatography (HPLC).(4) Using the recombinant inbred line population, thirteen additive QTLs for soluble oligosaccharides contents were detected on 11 chromosomes, 1B、 1D、2B、2D、3B、4A、5A、5D、6B and 7B, with a single QTL explaining 4.97%-53.57% of phenotypic variations.
        

小麦遗传图谱的加密和籽粒可溶性糖含量的QTL分析

摘要8-9
ABSTRACT9-10
1 前言11-32
    1.1 SSR 标记及其应用11-19
        1.1.1 SSR 的类型及分布12-13
        1.1.2 SSR 的变异与进化13-14
        1.1.3 SSR 标记的开发14
        1.1.4 SSR 标记的应用14-19
    1.2 分子标记遗传图谱19-22
        1.2.1 遗传作图群体19-21
        1.2.2 小麦分子标记遗传图谱21-22
    1.3 QTL 分析及其在小麦中应用22-29
        1.3.1 QTL 作图方法23-26
        1.3.2 小麦籽粒组分及其QTL 定位26-29
    1.4 小麦籽粒可溶性糖29-30
        1.4.1 可溶性糖与淀粉产量的关系29
        1.4.2 可溶性糖与种子活性29-30
        1.4.3 可溶性糖对小麦加工食品的影响30
    1.5 本研究的目的和意义30-32
2 材料与方法32-38
    2.1 试验材料32
        2.1.1 植物材料32
    2.2 试验方法32-38
        2.2.1 SSR 的查找32
        2.2.2 DNA 提取32-33
        2.2.3 SSR 标记分析33-36
        2.2.4 遗传作图36
        2.2.5 小麦籽粒可溶性糖含量的测定36-37
        2.2.6 小麦籽粒可溶性糖含量的相关分析37
        2.2.7 小麦籽粒可溶性糖含量的QTL 分析37-38
3 结果与分析38-51
    3.1 小麦遗传图谱的加密38-42
        3.1.1 SSR 引物的查找38
        3.1.2 SSR 引物的多态性检验38
        3.1.3 遗传作图38-42
    3.2 小麦籽粒可溶性糖含量的QTL 分析42-51
        3.2.1 可溶性糖在RIL 群体中的表现42-43
        3.2.2 简单相关分析43
        3.2.3 QTL 分析43-51
4 讨论51-55
    4.1 小麦分子标记遗传图谱51-52
    4.2 可溶性糖含量的QTL 分析52-53
    4.3 可溶性糖对小麦及加工食品品质的影响53-55
5 结论55-56
参考文献56-64
附录64-74
致谢74-75
攻读学位期间发表论文情况75
        下载全文需50


本文地址:

上一篇:小麦骨干亲本碧蚂4号及其姊妹系遗传差异分析
下一篇:适合棉花品种鉴定的SSR核心引物的筛选和品种鉴别

分享到: 分享小麦遗传图谱的加密和籽粒可溶性糖含量的QTL分析到腾讯微博           收藏
评论排行
公告