Broadening the applicability of CRISPR/Cas9 in plants

2018年第01期

关键词:
addressed;repeats;clustered;specificity;editing;RNA;chromosomes;finding;strict;surprising

摘要
     <正>The use of CRISPR(clustered regulatory interspaced short palindromic repeats)Cas9 is revolutionizing genome engineering—not only in plants(Schiml and Puchta,2016).This is due to the fact that with high specificity and efficiency,unique sequence motives can be addressed by the Cas9 nuclease.Most of its sequence specificity is defined by20 nt of a single guide(sg)RNA,which one can exchange


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